5FPW
proCathepsin B S9 from Trypanosoma congolense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 128.790, 223.220, 102.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.029 - 2.100 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21620 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.030 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.015 |
| Number of reflections | 85254 |
| Completeness [%] | 99.0 |
| Redundancy | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | THE RECOMBINANT PROTEIN (5.5 MG/ML) IN 20 MM NA-ACETATE, PH 4.5, 20 MM NACL IS CRYSTALLIZED BY THE HANGING DROP METHOD USING A PRECIPITANT SOLUTION MADE UP OF 0.2 M MES, PH 6.5, 5% (W/V) PEG 20000, 1.0 M SODIUM IODIDE. |
