5FP5
Structure of cyclin-dependent kinase 2 with small-molecule ligand 4- fluorobenzoic acid (AT222) in an alternate binding site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS |
Synchrotron site | SRS |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-05-02 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.677, 71.698, 72.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.010 - 2.160 |
R-factor | 0.1898 |
Rwork | 0.187 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hck |
RMSD bond length | 0.011 |
RMSD bond angle | 1.040 |
Data reduction software | d*TREK |
Data scaling software | DTSCALE |
Phasing software | AMoRE |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.160 |
Number of reflections | 14466 |
Completeness [%] | 93.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 10MM HEPES/NAOH PH7.4 15MM NACL PROTEIN CONCENTRATION: 8.6 MG/ML |