5FOT
HUMANISED MONOMERIC RADA IN COMPLEX WITH FHTU TETRAPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.220, 60.632, 87.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.296 - 1.189 |
| R-factor | 0.1337 |
| Rwork | 0.132 |
| R-free | 0.15670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.300 | 1.260 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 69123 | |
| <I/σ(I)> | 14.46 | 2.76 |
| Completeness [%] | 90.8 | 65.1 |
| Redundancy | 3.22 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
