5FOR
Cryptic TIR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 135.042, 135.042, 42.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.203 - 2.500 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.24410 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 15066 | |
| <I/σ(I)> | 25 | 7.2 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.5 1-10% PEG400 1.5-2.3 M AMMONIUM SULPHATE .001M I3C |
