5FOM
Crystal structure of the Cryptosporidium muris cytosolic leucyl-tRNA synthetase editing domain complex with the adduct AMP-AN6426
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.065, 65.065, 167.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.640 - 2.100 |
| R-factor | 0.20135 |
| Rwork | 0.199 |
| R-free | 0.23866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.667 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.640 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 21974 | |
| <I/σ(I)> | 29 | 5.6 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 10.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.4 M SODIUM/POTASSIUM PHOSPHATE PH 5.5. 20% GLYCEROL WAS USED AS CRYOPROTECTANT. |
