5FOH
Crystal structure of the catalytic domain of NcLPMO9A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.419, 80.419, 57.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.640 - 1.600 |
| R-factor | 0.15491 |
| Rwork | 0.154 |
| R-free | 0.17808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eir |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 1.010 |
| Number of reflections | 28860 | |
| <I/σ(I)> | 14 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG3350 0.2M LISO4, pH 6.5 |
