5FO7
Crystal Structure of Human Complement C3b at 2.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.580, 136.510, 140.930 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 36.808 - 2.800 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i07 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.670 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.070 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.990 |
| Number of reflections | 109359 | |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8% PEG 3350 35MM BIS-TRIS PH 5.5 |
