5FO5
Structure of the DNA-binding domain of Escherichia coli methionine biosynthesis regulator MetR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 27.760, 55.120, 49.990 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 8.921 - 2.160 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fxq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.529 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.920 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.120 | 0.180 |
| Number of reflections | 3976 | |
| <I/σ(I)> | 21.28 | 11.03 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 50 MM HEPES PH 7.2, 1.2 M AMMONIUM SULFATE, 200 MM POTASSIUM CHLORIDE, 50 MM MAGNESIUM CHLORIDE |
