5FO4
Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.700, 53.400, 63.000 |
| Unit cell angles | 88.90, 74.30, 89.70 |
Refinement procedure
| Resolution | 60.640 - 1.850 |
| R-factor | 0.19737 |
| Rwork | 0.195 |
| R-free | 0.25214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PLASMODIUM KNOWLESI LEURS EDITING DOMAIN |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.850 | 1.900 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 45297 | |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 87.0 | 84 |
| Redundancy | 1.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES (PH 7.5), 10% ISOPROPANOL, 20% (W/V) PEG 4000 AND 5% GLYCEROL |
