5FNY
Low solvent content crystal form of Zn containing Iron sulfur cluster repair protein YtfE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.024, 86.495, 51.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.170 - 2.010 |
| R-factor | 0.19094 |
| Rwork | 0.189 |
| R-free | 0.23476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fnn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.040 | 0.530 |
| Number of reflections | 26225 | |
| <I/σ(I)> | 18.11 | 2.42 |
| Completeness [%] | 93.4 | 75.2 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M BICINE PH9.0, 2.4M AMMONIUM REMARK 280 SULFATE, 2% PEG400 |
