5FN8
Crystal structure of rat CD45 extracellular region, domains d3-d4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.410, 131.160, 141.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.650 - 2.450 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.23570 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXD |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 2.520 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.960 |
| Number of reflections | 29113 | |
| <I/σ(I)> | 17.9 | 1.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 10% V/V 2-PROPANOL, 0.2M LISO4, 0.1M SODIUM PHOSPHATE-CITRATE, PH=4.2 |
