5FN6
Crystal structure of human CD45 extracellular region, domains d1-d3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-26 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.384, 132.384, 58.099 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.810 - 3.300 |
| R-factor | 0.2114 |
| Rwork | 0.209 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CD45 EXTRACELLULAR REGION D1-D2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.260 | 0.900 |
| Number of reflections | 5693 | |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% PEG8K, 0.2M (NH4)2SO4, 0.1M NA CACODYLATE, PH=6.5 |
