5FMR
crIFT52 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.765, 81.726, 120.476 |
| Unit cell angles | 90.00, 133.97, 90.00 |
Refinement procedure
| Resolution | 40.863 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23000 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 2.190 |
| High resolution limit [Å] | 1.900 | 2.000 |
| Rmerge | 0.070 | 0.810 |
| Number of reflections | 56947 | |
| <I/σ(I)> | 11.34 | 1.18 |
| Completeness [%] | 98.6 | 96.5 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM BIS-TRIS PH6.5 2 M AMMONIUM SULFATE |
