5FMQ
Crystal structure of the mid, cap-binding, mid-link and 627 domains from avian influenza A virus polymerase PB2 subunit bound to M7GTP H32 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 123.050, 123.050, 229.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.680 - 3.100 |
| R-factor | 0.27669 |
| Rwork | 0.276 |
| R-free | 0.29869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4WSB 4CB4 3KC6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.390 | 1.500 |
| Number of reflections | 11351 | |
| <I/σ(I)> | 8.21 | 2.02 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.8 | 10.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 0.1 M TRI-SODIUM CITRATE PH 5.8 AND 1 M AMMONIUM DI-HYDROGEN PHOSPHATE |
