5FMO
Crystal structure and proteomics analysis of empty virus like particles of Cowpea mosaic virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-03 |
| Detector | DECTRIS PILATUS3 6M |
| Spacegroup name | I 41 |
| Unit cell lengths | 655.970, 655.970, 571.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.359 |
| Rwork | 0.359 |
| R-free | 0.36200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ny7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.290 | 0.450 |
| Number of reflections | 3192363 | |
| <I/σ(I)> | 3.7 | 1.7 |
| Completeness [%] | 60.3 | 49.6 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 0.1M SODIUM ACETATE PH 4.7 3-4%(W/V)PEG 3350 0.3M AMMONIUM SULFATE 5% GLYCEROL |
