5FMN

The nickel-responsive transcriptional regulator InrS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Detector	PILATUS
Spacegroup name	I 2 2 2
Unit cell lengths	64.290, 77.790, 85.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.540 - 2.400
R-factor	0.20812
Rwork	0.204
R-free	0.28231
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.398
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.000
High resolution limit [Å]	2.400
Rmerge	0.040
Number of reflections	8308
<I/σ(I)>	27.7
Completeness [%]	95.0
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			HANGING DROP 100+ 100 NL WITH 0.2 M MGCL2, 0.1 TRIS PH 8.5 AND 20% PEG8000