5FM9
human Notch 1, EGF 4-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.260, 21.150, 83.560 |
| Unit cell angles | 90.00, 116.27, 90.00 |
Refinement procedure
| Resolution | 64.730 - 2.920 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VJ3.PDB |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.730 | 3.000 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.120 | 0.370 |
| Number of reflections | 5015 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 96.9 | 98 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PH 8.5 |
