5FM8
Structure of the C-terminally extended domain My4 of human myomesin (space group P65)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.410, 97.410, 106.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.360 - 2.050 |
| R-factor | 0.19364 |
| Rwork | 0.192 |
| R-free | 0.21687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fm5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.360 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.130 | 1.500 |
| Number of reflections | 35371 | |
| <I/σ(I)> | 13.4 | 1.6 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 21 % (W/V) PEG 2000 MME, 10 MM NICL2, 100 MM TRIS/HCL PH 8.8 |
