5FM7
Double-heterohexameric rings of full-length Rvb1(ADP)Rvb2(ADP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 208.770, 208.770, 138.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.632 - 2.901 |
| R-factor | 0.2598 |
| Rwork | 0.259 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fm6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.614 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 1.500 |
| Number of reflections | 25695 | |
| <I/σ(I)> | 11.5 | 0.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.3 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.0,1 M SUCCINATE, 1.2% MME |
