5FM5
Crystal structure of the myomesin:obscurin-like-1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.180, 134.380, 68.390 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 75.400 - 3.100 |
| R-factor | 0.21849 |
| Rwork | 0.216 |
| R-free | 0.25906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2NZI 2wp3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.190 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 14811 | |
| <I/σ(I)> | 12.7 | 1.8 |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG8000, 0.2M MGCL2, 0.1M TRIS-HCL, PH 8.5 |
