5FLY
The FhuD protein from S.pseudintermedius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.582, 53.822, 120.248 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 49.019 - 1.598 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 241352 | |
| <I/σ(I)> | 10.66 | 1.86 |
| Completeness [%] | 99.2 | 94.8 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M CDCL2 30 % V/V PEG 400 0.1 M NA ACETATE PH 4.6 |
