5FL3
PilT2 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.750, 106.750, 71.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.761 - 2.520 |
| R-factor | 0.1883 |
| Rwork | 0.185 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.760 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 15793 | |
| <I/σ(I)> | 19.9 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS/HCL PH 5.5, 1%(W/V) PEG 3350, 1.0 M AMMONIUM SULFATE |
