5FKS
Unraveling the first step of xyloglucan degradation by the soil saprophyte Cellvibrio japonicus through the functional and structural characterization of a potent GH74 endo-xyloglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-12-16 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.360, 127.690, 73.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.140 - 1.990 |
| R-factor | 0.16998 |
| Rwork | 0.168 |
| R-free | 0.21277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.853 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.130 | 0.580 |
| Number of reflections | 58267 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS (PH 7.8), 0.3 M POTASSIUM BROMIDE, 8 % PGA-LM |
