5FKR
Unraveling the first step of xyloglucan degradation by the soil saprophyte Cellvibrio japonicus through the functional and structural characterization of a potent GH74 endo-xyloglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-11-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.890, 131.910, 73.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.170 - 2.280 |
| R-factor | 0.21265 |
| Rwork | 0.210 |
| R-free | 0.26836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lgn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.658 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.170 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.130 | 0.620 |
| Number of reflections | 39506 | |
| <I/σ(I)> | 11.4 | 2.8 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS (PH 7.8), 0.3 M POTASSIUM BROMIDE, 8 % PGA-LM |
