5FKQ
Unraveling the first step of xyloglucan degradation by the soil saprophyte Cellvibrio japonicus through the functional and structural characterization of a potent GH74 endo-xyloglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.002, 94.116, 105.640 |
| Unit cell angles | 90.00, 103.27, 90.00 |
Refinement procedure
| Resolution | 102.820 - 1.710 |
| R-factor | 0.19408 |
| Rwork | 0.192 |
| R-free | 0.22462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.827 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.100 | 0.730 |
| Number of reflections | 172392 | |
| <I/σ(I)> | 11 | 1.6 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM ACETATE (PH 5.0), 0.6 M SODIUM FORMATE, 8 % W/V PGA-LM |
