5FKH
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is CU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2014-02-25 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.496, 62.496, 155.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.499 - 2.650 |
R-factor | 0.2329 |
Rwork | 0.231 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fjc |
RMSD bond length | 0.002 |
RMSD bond angle | 0.621 |
Data reduction software | HKL |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.500 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.030 | 0.550 |
Number of reflections | 9323 | |
<I/σ(I)> | 16.6 | 1.7 |
Completeness [%] | 98.7 | 99.7 |
Redundancy | 3.1 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 10 MM BACL2, 12 MM SPERMINE-HCL, 14% (V/V) MPD |