5FKH

SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is CU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	173
Detector technology	PIXEL
Collection date	2014-02-25
Detector	DECTRIS PIXEL
Spacegroup name	P 43 21 2
Unit cell lengths	62.496, 62.496, 155.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.499 - 2.650
R-factor	0.2329
Rwork	0.231
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fjc
RMSD bond length	0.002
RMSD bond angle	0.621
Data reduction software	HKL
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.500	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.030	0.550
Number of reflections	9323
<I/σ(I)>	16.6	1.7
Completeness [%]	98.7	99.7
Redundancy	3.1	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 10 MM BACL2, 12 MM SPERMINE-HCL, 14% (V/V) MPD