5FKG

SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is CG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	173
Detector technology	PIXEL
Collection date	2014-04-17
Detector	DECTRIS PILATUS3 6M
Spacegroup name	P 43 21 2
Unit cell lengths	61.252, 61.252, 155.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.013 - 2.950
R-factor	0.2371
Rwork	0.234
R-free	0.29870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fk3
RMSD bond length	0.005
RMSD bond angle	1.213
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.250	3.130
High resolution limit [Å]	2.950	2.950
Rmerge	0.160	1.500
Number of reflections	6751
<I/σ(I)>	9.7	1.9
Completeness [%]	100.0	100
Redundancy	9.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 15 MM BACL2, 12 MM SPERMINE-HCL, 8 % (V/V) MPD