5FKD
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is UA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.770, 61.770, 153.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.326 - 3.000 |
| R-factor | 0.2358 |
| Rwork | 0.233 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fk3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.610 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.770 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.190 | 1.500 |
| Number of reflections | 6437 | |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 30 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD |
