5FKA
Crystal structure of staphylococcal enterotoxin E in complex with a T cell receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.290, 150.143, 39.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.400 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4UDU 4udt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.780 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.680 |
| Number of reflections | 26772 | |
| <I/σ(I)> | 16.3 | 1.9 |
| Completeness [%] | 98.8 | 92.4 |
| Redundancy | 6.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 25% W/V PEG 3350, 0.1 M GLYCINE PH 9.0, 0.1 M NACL |
