5FK5
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is AA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2014-07-08 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.784, 61.784, 154.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.988 - 3.315 |
R-factor | 0.2043 |
Rwork | 0.199 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fjc |
RMSD bond length | 0.003 |
RMSD bond angle | 0.718 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.990 | 3.400 |
High resolution limit [Å] | 3.310 | 3.310 |
Rmerge | 0.140 | 1.370 |
Number of reflections | 4912 | |
<I/σ(I)> | 11.9 | 2.9 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 9.2 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 15 MM BACL2, 12 MM SPERMINE-HCL, 8% (V/V) MPD |