5FK3
SAM-I riboswitch bearing the H. marismortui Kt-7 variant 3bn is CC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2013-08-05 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.262, 62.262, 153.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.711 - 2.500 |
R-factor | 0.2095 |
Rwork | 0.206 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WWPDB ENTRY 5FK2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.677 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.890 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.090 | 0.090 |
Number of reflections | 11159 | |
<I/σ(I)> | 13.1 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.3 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 10 MM BACL2, 12 MM SPERMINE-HCL, 14% (V/V) MPD |