5FJR
N-acyl amino acid racemase from Amycolatopsis sp. Ts-1-60: Q26A M50I G291D F323Y mutant in complex with N-acetyl napthylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-12-07 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 217.670, 217.670, 263.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 107.890 - 2.440 |
| R-factor | 0.17453 |
| Rwork | 0.172 |
| R-free | 0.22052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6g |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.241 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.250 | 2.500 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.090 | 0.710 |
| Number of reflections | 88697 | |
| <I/σ(I)> | 17.7 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS HCL PH 8.0; 15% (W/V) PEG 4K; 800 MM SODIUM FORMATE; PROTEIN AT 8 MG PER ML |
