5FJN
Structure of L-Amino acid deaminase from Proteus myxofaciens in complex with anthranilate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-13 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.810, 93.320, 75.070 |
| Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
| Resolution | 41.190 - 1.750 |
| R-factor | 0.15842 |
| Rwork | 0.156 |
| R-free | 0.19669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.030 | 0.510 |
| Number of reflections | 96409 | |
| <I/σ(I)> | 14.9 | 1.9 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 15% PEG 6K, 0.1 M MES (PH 6.5), 10% MPD |
