5FJM
Structure of L-Amino acid deaminase from Proteus myxofaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.760, 93.710, 74.690 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 46.850 - 2.000 |
| R-factor | 0.17855 |
| Rwork | 0.177 |
| R-free | 0.20898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gag |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.080 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 65982 | |
| <I/σ(I)> | 12 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 15% PEG 6K, 0.1 M MES (PH 6.5), 10% MPD |
