5FJJ
Three-dimensional structures of two heavily N-glycosylated Aspergillus sp. Family GH3 beta-D-glucosidases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 139.020, 141.463, 193.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 114.430 - 1.950 |
| R-factor | 0.21764 |
| Rwork | 0.216 |
| R-free | 0.25486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fji |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.632 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.230 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.140 | 0.840 |
| Number of reflections | 271085 | |
| <I/σ(I)> | 17.1 | 1.9 |
| Completeness [%] | 98.4 | 94.9 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH 7.5, 0.2M MAGNESIUM CHLORIDE, 30% PEG 400 |
