5FJG
The crystal structure of light-driven chloride pump ClR in pH 4.5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 77.027, 49.434, 77.912 |
| Unit cell angles | 90.00, 96.94, 90.00 |
Refinement procedure
| Resolution | 38.670 - 2.000 |
| R-factor | 0.1696 |
| Rwork | 0.167 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.670 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.330 |
| Number of reflections | 19868 | |
| <I/σ(I)> | 14.53 | 5.15 |
| Completeness [%] | 99.2 | 97.3 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | PH 4.5 |
