5FJC
SAM-I riboswitch bearing the H. marismortui Kt-7 variant C-2bU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.398, 60.398, 152.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.134 - 1.710 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b5r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.122 |
| Data reduction software | ESRF (AUTOPROCESS) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.130 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.050 | 0.910 |
| Number of reflections | 31465 | |
| <I/σ(I)> | 20.6 | 1.2 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 9.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 40 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD. |
