5FIT
FHIT-SUBSTRATE ANALOG
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1996-09-08 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.640, 50.640, 267.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 25.600 * |
| Data reduction software | MADNES |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.033 |
| Total number of observations | 71374 * |
| Number of reflections | 17347 |
| Completeness [%] | 95.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | Lima, C.D., (1997) Structure (London), 5, 763. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | AmSO4 | 1.25-1.35 (M) | |
| 3 | 1 | 1 | sodium cacodylate | 100 (mM) |
