5FIN
DARPins as a new tool for experimental phasing in protein crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-24 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.540, 50.870, 93.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.500 - 2.340 |
| R-factor | 0.2313 |
| Rwork | 0.229 |
| R-free | 0.27020 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 7716 | |
| <I/σ(I)> | 13.39 | 2.09 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.82 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 25% W/V PEG 6000, 0.1M HEPES, PH 8.0 |
