5FIG
APO-CSP3 (COPPER STORAGE PROTEIN 3) FROM BACILLUS SUBTILIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.087, 126.510, 85.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.740 - 1.700 |
| R-factor | 0.22085 |
| Rwork | 0.220 |
| R-free | 0.24262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5arm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.847 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.710 |
| Number of reflections | 64008 | |
| <I/σ(I)> | 8 | 1.5 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM NACL, 100 MM CITRATE PH 4, 800 MM AMMONIUM SULFATE |
