5FIE

Crystal structure of N-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-09-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 41 21 2
Unit cell lengths	88.615, 88.615, 48.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.307 - 2.000
R-factor	0.187
Rwork	0.185
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f44
RMSD bond length	0.019
RMSD bond angle	0.888
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	2.190
High resolution limit [Å]	2.000	2.000
Rmerge	0.135	0.645
Number of reflections	13657
<I/σ(I)>	11.7	3.7
Completeness [%]	99.9	99.8
Redundancy	6.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2M sodium formate, 20% PEG 3350