5FID
Crystal structure of the protein elicitor MoHrip2 from Magnaporthe oryzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.245, 43.511, 74.001 |
| Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
| Resolution | 31.883 - 1.809 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.20000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.151 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.288 |
| Number of reflections | 21184 | |
| <I/σ(I)> | 16.8 | 2.3 |
| Completeness [%] | 96.6 | 86.4 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2M ammonium sulfate |
