5FIA
Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-11 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.420, 71.820, 94.180 |
| Unit cell angles | 90.00, 111.96, 90.00 |
Refinement procedure
| Resolution | 48.805 - 1.750 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.19010 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.848 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.860 | |
| Number of reflections | 100375 | |
| <I/σ(I)> | 17.39 | 2.2 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, MPD 40% |
