5FHH
Structure of human Pif1 helicase domain residues 200-641
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 204.750, 204.750, 77.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.062 - 3.600 |
| R-factor | 0.3125 |
| Rwork | 0.310 |
| R-free | 0.35480 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.355 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.200 | 3.780 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 22636 | |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 94.9 | 87.6 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3% tacsimate, 0.1M Bis-Tris, 15% glycerol, 15% PEG8000 |
