5FHB

Crystal Structure of Protective Ebola Virus Antibody 100

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	71.540, 71.540, 199.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.487 - 1.973
R-factor	0.1602
Rwork	0.159
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 4QHK
RMSD bond length	0.018
RMSD bond angle	1.623
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.487	38.790	2.020
High resolution limit [Å]	1.970	9.040	1.970
Rmerge	0.134	0.043	0.751
Rpim	0.044	0.015	0.261
Total number of observations	443933	4398	26792
Number of reflections	42605
<I/σ(I)>	11.3	18.5	3.1
Completeness [%]	100.0	97.8	100
Redundancy	10.4	8.5	9.1
CC(1/2)	0.997	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	3.4mg/ml 100 Fab, 38% PEG 400, 4.75% PEG 3350, 0.1M sodium acetate pH 5.5