5FGY
Structure of human adenovirus 2 protease with cofactor pVIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | RIGAKU SATURN 70 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 112.420, 112.420, 49.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.360 - 2.100 |
| R-factor | 0.17663 |
| Rwork | 0.176 |
| R-free | 0.19691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.588 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.360 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.196 |
| Number of reflections | 20733 | |
| <I/σ(I)> | 16 | 6 |
| Completeness [%] | 97.7 | 95.7 |
| Redundancy | 5.54 | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277.15 | Grew over one week at 4 degrees Celsius in 0.1 M sodium citrate pH 6.4, 1.7 M sodium acetate, 1 mM DTT |
