5FGR
Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group with Yb Heavy atom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.28136 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.603, 75.252, 117.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.740 - 2.790 |
| R-factor | 0.19916 |
| Rwork | 0.197 |
| R-free | 0.23583 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.740 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.098 | 0.452 |
| Number of reflections | 13205 | |
| <I/σ(I)> | 22.7 | 7.1 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 14 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M MES, 0.02M zinc sulphate, 25% PEG 550MME, 50mM Yb-DTPA-BMA |
