5FGN

Integral membrane protein lipooligosaccharide phosphoethanolamine transferase A (EptA) from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	I 4 2 2
Unit cell lengths	187.285, 187.285, 205.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.821 - 2.750
R-factor	0.2155
Rwork	0.214
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kav
RMSD bond length	0.010
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	138.308	46.821	2.900
High resolution limit [Å]	2.750	8.700	2.750
Rmerge		0.034	2.366
Rmeas	0.278	0.037	2.488
Rpim	0.086	0.013	0.762
Total number of observations	487530	15544	70794
Number of reflections	47424
<I/σ(I)>	9.3	34.4	1.3
Completeness [%]	100.0	99.3	100
Redundancy	10.3	9.5	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium Sulphate, Hepes, Beta octylglucoside