5FGJ
Structure of tetrameric rat phenylalanine hydroxylase, residues 1-453
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.768, 102.686, 202.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.480 - 3.600 |
| R-factor | 0.2702 |
| Rwork | 0.268 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.563 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.690 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 1.287 | |
| Number of reflections | 24188 | |
| <I/σ(I)> | 6.5 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 25% PEG 3350, 0.2 M magnesium chloride, 0.1 M bis-tris:HCl |
