5FG9
Yeast 20S proteasome beta2-T(-2)V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.570, 301.550, 145.130 |
| Unit cell angles | 90.00, 113.36, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.20987 |
| Rwork | 0.209 |
| R-free | 0.22994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.869 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.457 |
| Number of reflections | 317541 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.1 | 93.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
